Description

Mutant T7 RNA+DNA polymerase is a recombinant form of T7 RNA polymerase with a single Y639F mutation that enables T7 RNA+DNA polymerase to incorporate 2´-deoxyribonucleoside triphosphates (dNTPs) into full-length transcripts more efficiently¹. The enzyme retains the same catalytic activity for incorporation of canonical NTPs and the same promoter specificity as wild-type T7 RNA polymerase.  This mutant can incorporate a vareity of 2´-modified-dNTPs such as 2´-fluorine or 2´-amino groups and enables efficient in vitro synthesis of modified RNA aptamers can use both single and double stranded templates.

1. Sousa, R. and Padilla, R. (1995) EMBO J. 14, 4609.